Intelligent multifunctional ruthenium monoatomic/ZnAl-LDH photocatalysts for simultaneous detection and rapid degradation of antibiotics.

The construction and precise synthesis of materials based on functional and structural orientations have emerged as a pivotal platform in the field of environmental management. In this paper, an efficient and stable catalyst (RuLDH) was constructed to achieve this goal. RuLDH comprises individual Ru atoms that are uniformly dispersed on ZnAl-LDH, achieved by room temperature stirring. Remarkably, RuLDH exhibits exceptional performance under visible light, effectively triggering the photocatalytic degradation of tetracycline hydrochloride (TC) via peroxymonosulfate (PMS) with a remarkable efficiency of 100%, all while avoiding the generation of highly toxic intermediates. In addition, RuLDH0.2 demonstrated its utility in fluorescence detection of TC, showcasing commendable analytical performance characterized by rapid response, low detection limit, and robust resistance to environmental interferences (with a detection limit of 1.0 mg/L). Notably, the RuLDH0.2/PMS/Vis system exhibited remarkable efficacy in treating actual pesticide wastewater, effectively exerting bactericidal and disinfectant effects. This study serves as a source of inspiration for the design of multifunctional single-atom catalysts, thereby pushing the boundaries of "integration of diagnosis and treatment" in environmental management and control.

Microplastics induced the differential responses of microbial-driven soil carbon and nitrogen cycles under warming.

The input of microplastics (MPs) and warming interfere with soil carbon (C) or nitrogen (N) cycles. Although the effects of warming and/or MPs on the cycles have been well studied, the biological coupling of microbial-driven cycles was neglected. Here, the synergistic changes of the cycles were investigated using batch incubation experiments. As results, the influences of MPs were not significant at 15, 20, and 25 °C, and yet, high temperature (i.e., 30 °C) reduced the respiration of high-concentration MPs-amended soil by 9.80%, and increased dissolved organic carbon (DOC) by 14.74%. In contrast, high temperature did not change the effect of MPs on N. The decrease of microbial biomass carbon (MBC) and the constant of microbial biomass nitrogen (MBN) indicated that microbial N utilization was enhanced, which might be attributed to the enrichments of adapted populations, such as Conexibacter, Acidothermus, and Acidibacter. These observations revealed that high temperature and MPs drove the differential response of soil C and N cycles. Additionally, the transcriptomic provided genomic evidence of the response. In summary, the high temperature was a prerequisite for the MPs-driven response, which underscored new ecological risks of MPs under global warming and emphasized the need for carbon emission reduction and better plastic product regulation.

New insights into atomic layer BiOBr quantum dot/ZnAl-LDH composites in photocatalytic water treatment: Performance and mechanism.

Insufficient absorption sites and low charge separation notably limit the activation of photocatalytic molecular oxygen. In this study, atomic-layer BiOBr (BiOBr-QDs)/ZnAl-LDH composites with a considerable number of edges were developed to address the above-mentioned problems. The result of this study indicated the spatial separation of atomic-layer BiOBr-QDs/ZnAl-LDH's conduction band maximum (CBM) and valence band minimum (VBM). As a result, holes were produced on the substrate surface based on irradiation, and electrons were generated at the sites of the edge, such that ultra-fast charge separation can be carried out. The edges exposed massive adsorption sites in terms of oxygen molecules. Thus, electrons at the sites of the edge led to the reduction of absorbed oxygen molecules, thus exhibiting stronger photocatalytic •O2- production. Furthermore, the result confirmed that the atomic layer BiOBr-QDs/ZnAl-LDH are promising in environmental catalytic degradation for its increased activity of •O2- production. In this study, a novel insight into advanced photocatalyst design based on edge unsaturated ligand engineering at an atomic level is provided.

Lysogenic bacteriophages encoding arsenic resistance determinants promote bacterial community adaptation to arsenic toxicity.

Emerging evidence from genomics gives us a glimpse into the potential contribution of lysogenic bacteriophages (phages) to the environmental adaptability of their hosts. However, it is challenging to quantify this kind of contribution due to the lack of appropriate genetic markers and the associated controllable environmental factors. Here, based on the unique transformable nature of arsenic (the controllable environmental factor), a series of flooding microcosms was established to investigate the contribution of arsM-bearing lysogenic phages to their hosts' adaptation to trivalent arsenic [As(III)] toxicity, where arsM is the marker gene associated with microbial As(III) detoxification. In the 15-day flooding period, the concentration of As(III) was significantly increased, and this elevated As(III) toxicity visibly inhibited the bacterial population, but the latter quickly adapted to As(III) toxicity. During the flooding period, some lysogenic phages re-infected new hosts after an early burst, while others persistently followed the productive cycle (i.e., lytic cycle). The unique phage-host interplay contributed to the rapid spread of arsM among soil microbiota, enabling the quick recovery of the bacterial community. Moreover, the higher abundance of arsM imparted a greater arsenic methylation capability to soil microbiota. Collectively, this study provides experimental evidence for lysogenic phages assisting their hosts in adapting to an extreme environment, which highlights the ecological perspectives on lysogenic phage-host mutualism.

Advancing charge carriers separation and transformation by nitrogen self-doped hollow nanotubes g-C3N4 for enhancing photocatalytic degradation of organic pollutants.

The rapid recombination of photogenerated electrons and holes, low utilization of visible light and weak oxidation capacity significantly limit the photocatalytic activity for the degradation of organic pollutants. Doping is used as a conventional strategy for regulating the electronic structure of photocatalysts to obtain a wider light absorption, but also suffers from the problems of reduced charge mobility and oxidation capacity, which is not conducive to photocatalytic degradation of pollutants. To address this issue, a nitrogen self-doped hollow nanotubes g-C3N4 (N-PCN) was synthesized by synergistic self-doping and quantum confinement effects. The N-PCN exhibits excellent efficiency in photocatalytic degradation of TC compared to the pristine g-C3N4. The synthesized N-PCN has a more positive conduction band minimum and can generate more photogenerated electrons to reduce oxygen to superoxide radicals. In addition, experimental and theoretical evidence shows that N-self-doping not only suppresses the recombination of photogenerated charge carriers but also facilitates the adsorption of oxygen molecules. Consequently, more superoxide radicals and singlet oxygen are generated through oxygen activation process.

Phage-host interactions: The neglected part of biological wastewater treatment.

In wastewater treatment plants (WWTPs), the stable operation of biological wastewater treatment is strongly dependent on the stability of associated microbiota. Bacteriophages (phages), viruses that specifically infect bacteria and archaea, are highly abundant and diverse in WWTPs. Although phages do not have known metabolic functions for themselves, they can shape functional microbiota via various phage-host interactions to impact biological wastewater treatment. However, the developments of phage-host interaction in WWTPs and their impact on biological wastewater treatment are overlooked. Here, we review the current knowledge regarding the phage-host interactions in biological wastewater treatment, mainly focusing on the characteristics of different phage populations, the phage-driven changes in functional microbiota, and the potential driving factors of phage-host interactions. We also discuss the efforts required further to understand and manipulate the phage-host interactions in biological wastewater treatment. Overall, this review advocates more attention to the phage dynamics in WWTPs.

The clinical population pharmacokinetics, metabolomics and therapeutic analysis of alkaloids from Alstonia scholaris leaves in acute bronchitis patients.

BACKGROUND: Capsule of alkaloids from leaf of Alstonia scholaris (CALAS) is a new investigational botanical drug (No. 2011L01436) for respiratory disease. Clinical population pharmacokinetics (PK), metabolomics and therapeutic data are essential to guide dosing in patients. Previous research has demonstrated the potential therapeutic effect of CALAS on acute bronchitis. Further clinical trial data are needed to verify its clinical efficacy, pharmacokinetics behavior, and influence of dosage and other factors. PURPOSE: To verify the clinical efficacy and explore the potential biomarkers related to CALAS treatment for acute bronchitis. MATERIALS AND METHODS: Oral CALAS was assessed in a randomized, double-blind, placebo-controlled trial. Fifty-five eligible patients were randomly assigned to four cohorts to receive 20, 40 or 80 mg, of CALAS three times daily for seven days, or placebo. Each CALAS cohort included 15 subjects, and the placebo group included 10 subjects. A population PK model of CALAS was developed using plasma with four major alkaloid components. Metabolomics analysis was performed to identify biomarkers correlated with the therapeutic effect of CALAS, and efficacy and safety were assessed based on clinical symptoms and adverse events. RESULTS: The symptoms of acute bronchitis were alleviated by CALAS treatment without serious adverse events or clinically significant changes in vital signs, electrocardiography or upper abdominal Doppler ultrasonography. Moreover, one compartment model with first-order absorption showed that an increase in aspartate transaminase will reduce the clearance (CL) of scholaricine, and picrinine CL was inversely proportional to body mass index, while 19-epischolaricine and vallesamine CL increased with aging. The serum samples from acute bronchitis patients at different time points were analyzed using UPLC-QTOF in combination with the orthogonal projection to latent structures-discriminant analysis, which indicated higher levels of lysophosphatidylcholines, lysophosphatidylethanolamines and amino acids with CALAS treatment than with placebo. CONCLUSION: This is the first study to evaluate the clinical efficacy and explored the potential biomarkers related to CALAS therapeutic mechanism of acute bronchitis by means of clinical trial combined the metabolomics study. This exploratory study provides a basis for further research on clinical efficacy and optimal dosing regimens based on pharmacokinetics behavior. Additional acute bronchitis patients and CALAS PK samples collected in future studies may be used to improve model performance and maximize its clinical value.

Cationic polyacrylamide alleviated the inhibitory impact of ZnO nanoparticles on anaerobic digestion of waste activated sludge through reducing reactive oxygen species induced.

The enrichment of zinc oxide nanoparticles (ZnO NPs) in waste activated sludge (WAS) has raised concerns about their potential impact on anaerobic digestion of WAS. To date, there is no information regarding how to attenuate the negative effects of ZnO NPs on WAS anaerobic digestion. In this study, it was found that the appropriate amount of cationic polyacrylamide (cPAM) could mitigate the toxicity of ZnO NPs. During short-term exposure, the supplement of 4.0 mg cPAM/g TSS significantly restored biochemical methane potential from 28.6% inhibition to 9.3% inhibition compared with the control digester (P < 0.01). The spiked cPAM promoted the solubilization and acidification stages by weakening the contact between ZnO NPs and anaerobes in anaerobic digestion process, thus providing abundant substance for sequent bio-utilization. In the long-term semi-continues operated reactor, the continuous replacement of cPAM (at 4.0 mg/g TSS) significantly strengthened the recovery of VS destruction rate (20.3% to 26.4%, P < 0.01) and the daily yield of methane (93.5 mL/d to 124.2 mL/d, P < 0.01). Consistent with the restored performance, the application of cPAM increased the total microbial communities and the relative abundances of dominant acidogens and methanogens. Further explorations showed decreased toxicity of ZnO NPs primarily attributed to the decline of reactive oxygen species (ROS) induced by ZnO NPs.

Effects and mechanisms of modified biochars on microbial iron reduction of Geobacter sulfurreducens.

Biochar was proved as an electron shuttle to facilitate extracellular electron transfer (EET) of electrochemically active bacteria (EAB); however, its underlying mechanism was not fully understood. In this study, we aimed to further explore how the regulation of surface functional groups of biochar would affect the microbial iron reduction process of Geobacter sulfurreducens as a typical EAB. Two modified biochars were achieved after HNO3 (NBC) and NaBH4 (RBC) pretreatments, and a control biochar was produced after deionized water (WBC) washing. Results showed that WBC and RBC significantly accelerated microbial iron reduction of G. sulfurreducens PCA, while had no effect in the final Fe (II) minerals (e.g., vivianite and green rust (CO32-)). Besides, Brunauer-Emmett-Teller (BET) surface area, electron spin resonance (ESR) and electrochemical measurements showed that larger surface area, lower redox potential, and more redox-active groups (e.g., aromatic structures and quinone/hydroquinone moieties) in RBC explained its better electron transfer performance comparing to WBC. Interestingly, NBC completely suppressed the Fe (III) reduction process, mainly due to the production of reactive oxygen species which inhibited the growth of G. sulfurreducens PCA. Overall, this work paves a feasible way to regulate the surface functional groups for biochar, and comprehensively revealed its effect on EET process of microorganisms.

The arsenic chemical species proportion and viral arsenic biotransformation genes composition affects lysogenic phage treatment under arsenic stress.

When temperate phages and their hosts have a consistent interest, they are considered reciprocal. Based on the bacterium-phage collaboration, lysogenic phage treatment is a promising method to resist pollution through lysogenic phage reshaping indigenous microbial communities to maintain their ecological function under environmental stress. However, the potential factors affecting the establishment of bacterium-phage collaboration are still poorly understood. Here, lysogenic phages carrying arsenic biotransformation genes (ABGs) were induced from the enriched arsenic-resistant microorganisms (from arsenic-contaminated sites). The diversity analysis of viral arsC and arsM demonstrated that arsM tended to proliferate rapidly under high arsenic levels, and the transduction of arsM might be the key to lysogenic phages to help the hosts relieve the stress of high arsenic. Microcosm experiments confirmed that with the increase of the As(III) content (0% to 50%, of 200 mg/kg total arsenic) in the soil, inoculation of lysogenic phages with both arsC and arsM resulted in more transduction of arsM (0.06 ± 0.007 to 0.23 ± 0.024 per 16S rRNA) among soil microorganisms. In contrast, inoculation of lysogenic phages carrying the only arsC transduces more arsC (0.12 ± 0.037 to 0.315 ± 0.051 per 16S rRNA) compare with the control. This article confirmed that different arsenic species proportions and different viral gene compositions could change the abundance of ABGs in the soil microbe, which might provide basic knowledge for further understanding of arsenic pollution control mediated by lysogenic phage.

Benzyl butyl phthalate activates prophage, threatening the stable operation of waste activated sludge anaerobic digestion.

The stable operation of the anaerobic digestion of waste activated sludge (WAS) is threatened by numerous emerging contaminants. Meanwhile, the extensive microplastic pollution increased the environmental exposure risk of plasticizer benzyl butyl phthalate (BBP), the BBP content has reached a substantial level in WAS. However, the effect of BBP on WAS anaerobic digestion is still unknown. Here we show that high-level BBP brings on anaerobic digestion upset. The presence of 10.0 mg/L BBP (in sludge with 17,640 ± 510 mg/L TSS) led to deferred cell lysis, which was confirmed by the results of continuous parallel factor analysis of dissolved organic matter and the liberation of lactate dehydrogenase. Further, the deferred cell rupture was confirmed associate with prophage activation during WAS anaerobic digestion. Besides solubilization, the hydrolysis, acetogenesis and methanogenesis were also affected by the addition of BBP. The long-term effects of BBP revealed that the dominant microbial structure in anaerobic digester was stable, but the abundance of many functional microorganisms was changed, including short chain fatty acid producers and consumers. This work highlights one of the susceptibility mechanisms for WAS anaerobic digestion processes and provides new perspectives for the comprehensive assessment of emerging contaminant's environmental risks.

The reduction of nitrobenzene by extracellular electron transfer facilitated by Fe-bearing biochar derived from sewage sludge.

In this work, the incorporation of Fe-bearing sludge-derived biochar greatly enhanced both biotic and abiotic reduction of nitrobenzene (NB) to aniline, which was attributed to the concomitant microbial dissimilatory iron reduction. Biogenic Fe(II) produced by Geobacter sulfurreducens dominated the anaerobic reduction of NB following the pseudo-first-order kinetic. Besides, the increase of pyrolysis temperature from 600 to 900 ℃ to generate biochar resulted in an accelerated removal rate of NB in Geobacter-biochar combined system. The morphology and structural characterization of biochar with G. sulfurreducens confirmed the formation of conductive bacteria-biochar aggregates. Electrochemical measurements suggested the presence of graphitized domains and quinone-like moieties in biochar as redox-active centers, which might play an important role in accelerating electron transfer for microbial dissimilatory iron reduction and NB degradation. This study provides a feasible way of using Fe-bearing sludge as a valuable feedstock for biochar generation and its application with electrochemically active bacteria for the bioremediation of nitroaromatic compounds-polluted wastewater.

Extracellular electron transfer leading to the biological mediated production of reduced graphene oxide.

To explore a green, low-cost, and efficient strategy to synthesis reduced graphene oxide (RGO), the process and mechanism of the graphene oxide (GO) reduction by a model electrochemically active bacteria (EAB), Geobacter sulfurreducens PCA, were studied. In this work, up to 1.0 mg mL-1 of GO was reduced by G. sulfurreducens within 0.5-8 days. ID/IG ratio in reduced product was similar to chemically RGO. After microbial reduction, the peak which corresponded to the reflection of graphene oxide (001) disappeared, while another peak considered as graphite spacing (002) appeared. The peak intensity of typical oxygen function groups, such as carboxyl C-O and >O (epoxide) groups, diminished in bacterially induced RGO comparing to initial GO. Besides, we observed the doping of nitrogen and phosphorus elements in bacterially induced RGO. In a good agreement with that, better electrochemical performance was noticed after GO reduction. As confirmed with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) analysis, the maximum value of peak currents of bacterially induced RGO were significantly higher than those of GO. Our results showed the electron transfer at microbial cell/GO interface promoted the GO reduction, suggesting a broader application of EAB in biological mediated production of RGO.

Shifts in short-chain fatty acid profile, Fe(III) reduction and bacterial community with biochar amendment in rice paddy soil.

Biochar, a valuable product from the pyrolysis of agricultural and forestry residues, has been widely applied as soil amendment. However, the effect of different types of biochar on soil microorganisms and associated biochemical processes in paddy soil remains ambiguous. In this study, we investigated the impact of biochars derived from different feedstocks (rice straw, orange peel and bamboo powder) on the dynamics of short-chain fatty acids (SCFAs), iron concentration and bacterial community in paddy soil within 90 days of anaerobic incubation. Results showed that biochar amendment overall inhibited the accumulation of SCFAs while accelerating the Fe(III) reduction process in paddy soil. In addition, 16S rRNA gene sequencing results demonstrated that the α-diversity of the bacterial community significantly decreased in response to biochar amendments at day 1 but was relatively unaffected at the end of incubation, and incubation time was the major driver for the succession of the bacterial community. Furthermore, significant correlations between parameters (e.g. SCFAs and iron concentration) and bacterial taxa (e.g. Clostridia, Syntrophus, Syntrophobacter and Desulfatiglans) were observed. Overall, our findings demonstrated amendment with different types of biochar altered SCFA profile, Fe(III) reduction and bacterial biodiversity in rice paddy soil.

Metagenomic analysis reveals the effects of long-term antibiotic pressure on sludge anaerobic digestion and antimicrobial resistance risk.

Continuous stirred-tank digesters with tetracyclines and sulfonamides were operated to investigate the impacts of antibiotic pressure on sludge anaerobic digestion. The versatile methanogen Methanosarcinales and strictly hydrogenotrophic methanogen Methanobacteriales increased and decreased by 21.1% and 10.9% under antibiotic pressure, respectively. KEGG analysis revealed that hydrogenotrophic and acetoclastic methanogenesis pathways were all affected. The decrease in abundance of function genes involved in lipid metabolism, carbohydrate metabolism, and fatty acid degradation, would lead to a reduction in methane production by 25%. Network analysis indicated positive associations among tetracycline residuals, abundance of resistance genes (ARGs), and specific member of potential hosts. Over 1000 ARG subtypes were widely detected in sludge, including macrolide (28%), tetracycline (24%), fluoroquinolone (20%), and peptide (20%) resistance genes. AD process exposed to long-term antibiotic would increase the diversity and abundance of ARG, enhance the association of ARG with specific microbes, and select bacteria able to perform chemotaxis mechanism.

Chromosomal expression of CadR on Pseudomonas aeruginosa for the removal of Cd(II) from aqueous solutions.

Genetically engineered bacteria for pollution control of heavy metal have been widely studied, however, using Pseudomonas aeruginosa (P. aeruginosa) that can adapt to various circumstances to remediate heavy metal pollution is rarely reported. In this study, we employed CadR, a cadmium (Cd)-specific binding protein, displaying on the surface of P. aeruginosa with chromosomal expression. The genetically engineered (GE) P. aeruginosa still flourished in the 30th generation in the LB broth which contained 100 µM Cd(II), exhibiting an excellent genetic stability. Chromosomally expressed P. aeruginosa showed an adsorption capacity of up to 131.9 µmol/g of Cd(II). In addition, the low concentration of the coexisting two valence ions has no significant effect on adsorption capacity of Cd(II). This study provides a direction for application of P. aeruginosa in environment remediation.

Organic loading rate and hydraulic retention time shape distinct ecological networks of anaerobic digestion related microbiome.

Understanding of how anaerobic digestion (AD)-related microbiomes are constructed by operational parameters or their interactions within the biochemical process is limited. Using high-throughput sequencing and molecular ecological network analysis, this study shows the succession of AD-related microbiome hosting diverse members of the phylum Actinobacteria, Bacteroidetes, Euryarchaeota, and Firmicutes, which were affected by organic loading rate (OLR) and hydraulic retention time (HRT). OLR formed finer microbial network modules than HRT (12 vs. 6), suggesting the further subdivision of functional components. Biomarkers were also identified in OLR or HRT groups (e.g. the family Actinomycetaceae, Methanosaetaceae and Aminiphilaceae). The most pair-wise link between Firmicutes and biogas production indicates the keystone members based on network features can be considered as markers in the regulation of AD. A set of 40% species ("core microbiome") were similar across different digesters. Such noteworthy overlap of microbiomes indicates they are generalists in maintaining the ecological stability of digesters.

Rapid startup of thermophilic anaerobic digester to remove tetracycline and sulfonamides resistance genes from sewage sludge.

Spread of antibiotic resistance genes (ARGs) originating from sewage sludge is highlighted as an eminent health threat. This study established a thermophilic anaerobic digester using one-step startup strategy to quickly remove tetracycline and sulfonamides resistance genes from sewage sludge. At least 20days were saved in the startup period from mesophilic to thermophilic condition. Based on the results of 16S rDNA amplicons sequencing and predicted metagenomic method, the successful startup largely relied on the fast colonization of core thermophilic microbial population (e.g. Firmicutes, Proteobacteria, Actinobacteria). Microbial metabolic gene pathways for substrate degradation and methane production was also increased by one-step mode. In addition, real-time quantitative PCR approach revealed that most targeted tetracycline and sulfonamides resistance genes ARGs (sulI, tetA, tetO, tetX) were substantially removed during thermophilic digestion (removal efficiency>80%). Network analysis showed that the elimination of ARGs was attributed to the decline of their horizontal (intI1 item) and vertical (potential hosts) transfer-related elements under high-temperature. This research demonstrated that rapid startup thermophilic anaerobic digestion of wastewater solids would be a suitable technology for reducing quantities of various ARGs.

[Evaluation of implementation effect of clinical practice guideline on traditional Chinese medicine therapy alone or combined with antibiotics for acute tonsillitis].

The purpose of this article is to evaluate the clinical effect of clinical practice guideline on traditional Chinese medicine therapy alone or combined with antibiotics for acute tonsillitis. The applicability and application of the Guideline were evaluated based on the clinicians by using the electronic questionnaire. Questionnaires about 538 on application evaluation and 502 questionnaires on applicability evaluation were completed from April 28 to July 9, 2018. The subjects in the questionnaires include the clinicians with junior title, intermediate title, and senior title that have used this Guideline. The descriptive statistical analysis of the collected questionnaire was carried out. In the applicability evaluation, according to the classification of professional titles, the application rate was highest in intermediate title clinicians (26.77%), followed by junior (23.98%) and deputy senior (19.33%) professional title clinicians. In the quality evaluation, the rationality of application scope (98.61%) and the terminology accuracy (98.81%) scores were higher, and the rationality of differentiation and classification (96.05%) was the lowest. The applicability evaluation suggested that clinicians believed this Guideline had high safety (98.42%), reasonable content (98.03%), significant effect (99.6%), reduced use of antibiotics in Western medicine (93.89%), and a high applicability ratio (96.44%). In the application evaluation, Department of lung disease showed the highest application rate (44.24%); rationality of the Guideline was more than 97% in treatment rules and prophylaxis except the syndrome differentiation (92.75%); a high ratio of clinicians believed the recommended scheme was good: curative effect 97.4%, safety 97.59%, and economy 93.87%. The study shows that the clinical practice guideline on traditional Chinese medicine therapy alone or combined with antibiotics for acute tonsillitis is of good quality, high clinical use and good effect. It can be used as a standardized treatment scheme for acute tonsillitis in traditional Chinese medicine. But there are some unsuitable contents and need to be further improved. The Guideline should strengthen the revision on differentiation of symptoms and signs as well as prophylaxis.